In vitro tox assays for Medchem Support



Toxicity is the major cause of drug candidate failure in preclinical and clinical development stage, and also the major reason for the withdrawal of approved drugs from the market. While toxicity test has been traditionally completed in the preclinical phase, in vitro toxicity studies in the early drug discovery stages could significantly reduce failures at a later stage and prevent economic loss.


WuXi AppTec offers a panel of in vitro toxicity assays designed to identify compound toxicity in the early drug discovery stage. By utilizing cutting-edge technologies including conventional and automated patch clamp and high content screening (HCS), we provide the high-quality data quickly and cost-effectively. Applying these assays to your lead ID and optimization strategy helps to make a more thorough analysis of the severity and specificity of toxicity, then bto guide candidate compounds through the planning and execution of downstream in vivo toxicity and efficacy tests.





  • Cardiac ion channel safety panel (CIPA compliant) with manual and automated patch-clamp: including hERG, hCav1.2, hNav1.5 (early and late), hKCNQ1/mink, hKv4.3/KChIP2.2, hKir2.1 channels
  • Human stem cell derived cardiomyocytes cardiotoxicity testing with MEA



General Cytotoxicity


  • Cell viability Assay: CellTiter-Glo® (Promega) or HCS assay
  • Apoptosis assay
  • CellTox™ Green cytotoxicity assay (Promega)
  • ApoTox-Glo™ Triplex Assay (Promega)



Mitochondrial Toxicity


  • Mitochondrial Membrane Potential Assay
  • Mitochondrial Reactive Oxygen Species (ROS) Assay
  • MitoXpress® Xtra OCR Assay (HS method) (Luxcel)
  • MitoXpress® Cellular Energy Flux OCR/ECAR Assay from (Luxcel)
  • Glucose/Galactose Assay
  • MitoBiogenesis Assay





  • Phospholipidosis and steatosis assays



Hepatotoxicity with HepG2 or Primary Human Liver Cell Culture


  • Selected general cytotoxicity and mitochondrial toxicity assays listed above



Nephrotoxicity with HK-2 Cells


  • Selected general cytotoxicity and mitochondrial toxicity assays listed above





  • Ames assay
  • In vitro micronucleus assay



Photo Toxicity


  • In vitro 3T3 NRU Phototoxicity test


Fig 1. A hERG current recorded on QPatch, B hiPSC-vCMs recording on MEA system.

Fig 2, Mitochondrial membrane potential assay using HepG2 cells.

Fig 3. Nephrotoxicity assay with HK-2 cells. 

Fig 4. In vitro 3T3 NRU phototoxicity test


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