Identifying New Chemical Matter via High Throughput Screening (HTS)
State-of-the-art Equipment for HTS Support
Automation system
- Tecan
- POD and Echo
- JANUS
- BRAVO
- Platemate Plus
Plate washers
- Elx405 and Elx406
- DropArray Washing StationPlate Sealers
Readers to support HTS
- BD Biosciences Flow Cytometer with HTS mode
- Envision (with stacker)
- EnSpire2300
- FlexStation III
- Bioplex 200 Luminex
- FLIPR and Hamammatsu
- Topcount and Microbeta2
- Q-Patch and Ion Works
- Acumen and Odyssey (Li-COR)
HTS Compound Collection
Majority of the collection is semi-exclusive, non-commercially available
Selected from >550K compounds based on diversity, properties, PAINS* & Vacca-Reos# structural filters, reviewed by medicinal chemists, and Rule-of-5 Multi Parameter Optimization (MPO)
Total 167k compounds were collected and another 50k compounds expansion in progress
Arrayed in 384 plate ready for HTS
Stocks and powders available in China
HTS Collection (166K) - Property Distributions
WuXi HTS Case Study
Over 40 HTS successfully completed with WuXi HTS library.
Multiple HTS projects have successfully identified hits for further lead identification and optimization.
Targets cover divers fields
- Epigenetic targets
- GPCRs and nuclear receptors
- Kinases
Example of primary screening QC data
- Z’ value of plates in primary screening
- IC50 result of reference compound in cherry picking screening
